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Get. The invention of new drugs that inhibit endothelial exocytosis may prove useful in treating antibody-induced inflammatory disorders. Materials and MethodsMaterials. The antibody to HLA W6/32 was purchased from Sigma?5 M BCEF-A M (2 ,7 -bis-(2-carboxyethyl)-5-(and-6)carboxyfluorescein, acetoxymethyl ester), washed with Hanks' balanced salt solution (HBSS), and then added to HAEC. The cells wer
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Racellular functions such as protease cascades, extracellular morphogenetic peptides and secondary metabolism (Akanuma et al., 2009; Chater et al., 2010; Higo et al., 2012), but also contributing to the regulation of DnaA-mediated chromosome replication initiation (Wolanski et al., 2012). In S. griseus, many hundreds of direct targets for AdpA have been defined, and it is suspected that the unusua
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Get. The invention of new drugs that inhibit endothelial exocytosis may prove useful in treating antibody-induced inflammatory disorders. Materials and MethodsMaterials. The antibody to HLA W6/32 was purchased from Sigma?5 M BCEF-A M (2 ,7 -bis-(2-carboxyethyl)-5-(and-6)carboxyfluorescein, acetoxymethyl ester), washed with Hanks' balanced salt solution (HBSS), and then added to HAEC. The cells wer
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Ribed above except that only one tissue was tested for efficiency, and standard curves were approximately in the range of the sample values. All technical replicates were within 0.5 Ct for 224/228 combinations. Primer thermal regimes for these genes were reported previously [33].Data accessibilityGene expression data files have been uploaded to Gene Expression Omnibus (GSE48337).Additional filesAd
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This general class were present in the ur-actinobacterium, giving rise to the possibility of subtle control of sigma factor activity by signals that might include morphological checkpoints (as in the case of the spoIIAA/spoIIAB genes of B. subtilis; PiggotHilbert, 2004) or stress (as in the case of sigB of B. subtilis; Price, 2000). Indeed, BldG influences the activity of the stress-responsive sig
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On of genes for morphological differentiation and antibiotic production during vegetative growth and connecting the regulons of other regulators of these processes (den Hengst et al., 2010). BldD orthologues in simpler actinomycetes might well have roles both during growth, to repress functions associated with entry into stationary phase, and in stationary phase, in coordinating the expression of
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Ected p-value 0.05 and fold change 1.5. In experiments with a time component (e.g., Atlantic or pink salmon anterior kidney), a 2-way ANOVA was used to detect probes with a significant effect of infection and those with a time-infection interaction effect. Probes with a main effect but no interaction effect were filtered to retain only those that varied by 1.5 fold between control and experiment
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Ected p-value 0.05 and fold change 1.5. In experiments with a time component (e.g., Atlantic or pink salmon anterior kidney), a 2-way ANOVA was used to detect probes with a significant effect of infection and those with a time-infection interaction effect. Probes with a main effect but no interaction effect were filtered to retain only those that varied by 1.5 fold between control and experiment
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